Journal: BMC Cancer

Article Title: Viral-mediated oncolysis is the most critical factor in the late-phase of the tumor regression process upon vaccinia virus infection

doi: 10.1186/1471-2407-11-68

Figure Lengend Snippet: MHCII-positive immune cells, T-, B-, and NK cells did not significantly contribute to VACV-mediated tumor regression . GI-101A tumor-bearing mice were intraveneously (i.v.) injected with either 5 × 10 6 pfu/100 μl GLV-1h68 (a-e) or 1 × 10 6 pfu/100 μl GLV-1h68 (f-h) or PBS as a control. Immunosuppression was reached using 100/140 mg/kg cyclophosphamide monohydrate. (a) Fluorescence intensity measurement of recruited MHCII + leukocytes in GLV-1h68 infected GI-101A tumors treated or untreated with CPA. The fluorescence signal was measured in 4 images of each tumor (n = 5). Shown are the mean values +/- standard deviations. (b) Tumor growth was monitored weekly by measuring the tumor volume of five mice in each group. Shown are the mean values +/- standard deviations. The study was repeated in an independent experiment. CPA treatment was started 10 days p.i. (arrow). (c-e) Distribution of GLV-1h68 within the tumor tissue 42 days p.i. was visualized by GFP in whole tumor cross-sections of 5 different tumors; the corresponding 8-bit grey-scale images of GFP and nuclei were used for calculation of the extent (%) of viral infection in tumor cross-sections using ImageJ software. Shown are the mean values +/- standard deviations. (d, e) Representative images of CPA-treated GLV-1h68-infected tumor (d) compared to untreated GLV-1h68-infected tumor (e) revealed a higher degree of oncolytic tissue destruction. (f-h) Tumor growth in athymic nude Foxn1 nu (f), B6.12956- Rag2 tm1Fwa N12 (g), and Tac:NIHS- Lyst bg Foxn1 nu Btk xld (h) mice was monitored weekly by measuring the tumor volume of four to five mice in each group. In all models mice were intraveneously (i.v.) injected with 1 × 10 6 pfu/100 μl GLV-1h68 at day 39 post tumor cell implantation. Shown are the mean values + standard deviations. The asterisk (a, c) indicate a significant difference between experimental groups (* P < 0.05, *** P < 0.001; Student's t test). Scale bars represent 5 mm.

Article Snippet: Six-week-old female athymic nude Foxn1 nu mice were obtained from Harlan Winkelmann GmbH (Borchen, Germany).

Techniques: Injection, Control, Fluorescence, Infection, Software

Journal:

Article Title: Traveling stripes on the skin of a mutant mouse

doi: 10.1073/pnas.1731184100

Figure Lengend Snippet: Two types of aberrant alternative splicing between exons 6 and 7 of Foxn1tw mRNA. (Top) Normal splicing between exons 6 and 7 of Foxn1 mRNA. The nucleotide numbers of 5′ and 3′ of intron 6 are 39651 and 43956, respectively. The nucleotide numbers are referred to in the reported M. musculus whn gene sequence, GenBank accession no. Y12488. (Middle) The major form of the mutant mRNA. This type, 77% of the total of Foxn1tw mRNA, has a 54-bp deletion 5′ in exon 7 (43957–44010), generating an 18-aa deletion in the center of the DNA binding domain. (Bottom) The minor form of the mutant mRNA. This type, 23% of the total of Foxn1tw mRNA, has a 44-bp cryptic exon (40541–40584) and a 34-bp deletion 5′ in exon 7 (43957–43991), generating a translational termination (43992–43994).

Article Snippet: The mutant male mice were crossed with the albino KSN nude ( foxn1 nu / foxn1 nu ) mice purchased from Japan SLC (Hamamatsu, Japan).

Techniques: Sequencing, Mutagenesis, Binding Assay